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Background: c-Kit is a transmembrane tyrosine kinase encoded by the cKit proto oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. Phosphorylation at tyrosine 721 of c-Kit allows binding and activation of PI3 kinase.

Host species/Isotype: Rabbit/IgG

Species reactivity: Human, Mouse

Immunogen: KLH conjugated synthetic peptide derived from human c-Kit/CD117.

Storage instructions:Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Concentration: 1mg/ml

Purity: affinity purified by Protein A

Molecular weight: 105KDa

Clonality: Polyclonal

Tested applications:

WB=1:500-2000

ELISA=1:500-1000

IHC-P=1:100-500

IHC-F=1:100-500

Flow-Cyt=1μg /test

ICC=1:100-500

IF=1:100-500