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商品編號


AG-CS1-0100-M001



品牌


Adipogen



公司


Adipogen



公司分類


Cancer



Size

1 mg


商品信息


More Information



Product Details



Synonyms

pro-Tosyl-L-Arginine Methyl Ester



Product Type

Chemical



Properties



Formula

C
34
H
38
N
4
O
12
S



MW

726.8



CAS

1362911-19-0



Purity Chemicals

≥90%



Formulation

20mM stock solution in DMSO.



Declaration

Manufactured by Boston Biochem



Other Product Data


Use:
When diluting proTAME into the cell growth medium, it is best to add the DMSO stock solution to an empty tube first, and then add a large volume of growth medium followed by immediate mixing. proTAME is sparingly soluble in aqueous solutions. To avoid precipitation, instant and highly effective mixing is necessary. Optimal dose for each cell line needs to be established empirically due to cell-line specific differences in prodrug activation. HeLa and hTERT-RPE1 cells activate the prodrug efficiently, with mitotic arrest observed at 12?M compound. MCF10a cells activate the prodrug much less efficiently.



InChi Key

MHYOVHULCQSDRZ-NDEPHWFRSA-N



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

-20°C



Long Term Storage

-20°C



Handling Advice

Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 6 months after receipt when stored at -20°C.
Stock solutions are stable for at least 3 months when stored at -20°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF






Cell permeable prodrug capable of inducing mitotic arrest and cell death in HeLa and other cell types.

Inhibitor of the ubiquitin ligase activity of the anaphase-promoting complex/cyclosome (APC/C) by preventing its activation by Cdc20 and Cdh1.

proTAME is converted to its active parent molecule (TAME; Tosyl-L-arginine methyl ester) by intracellular esterases.



Product References

Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage: X. Zeng, et al.; Cancer Cell
18,
382 (2010)

Cohesion fatigue explains why pharmacological inhibition of the APC/C induces a spindle checkpoint-dependent mitotic arrest: P. Lara-Gonzalez & S.S. Taylor; PLoS One
7,
e49041 (2012)

Targeting mitotic exit with hyperthermia or APC/C inhibition to increase paclitaxel efficacy: S. Giovinazzi, et al.; Cell Cycle
12,
2598 (2013)

Targeting the anaphase-promoting complex/cyclosome (APC/C)- bromodomain containing 7 (BRD7) pathway for human osteosarcoma: K. Hu, et al.; Oncotarget
5,
3088 (2014)