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商品編號


3410-4APW-2



品牌


Mabtech



公司


Mabtech



公司分類(lèi)


Human/NHP



Size

2 plates














商品信息
ELISpot
PLUS
for enumeration of cells secreting IL-4
This kit is ideal for users who want a convenient and sensitive assay. The assay is designed for the enumeration of cells secreting human IL-4. The kit includes ELISpot plates pre-coated with monoclonal antibody, biotinylated detection antibody, Streptavidin-ALP and ready-to-use BCIP/NBT-plus substrate. A polyclonal activator for human T cells (mAb CD3-2) is supplied as a positive control for cytokine secretion.
Pre-coated plates reduce assay time and minimize vari
ABI
lity.
Human IL-4 ELISpot
PLUS
kits are also available with Streptavidin-HRP and TMB substrate. See Related products.
Species cross-reactivity
The monoclonal antibodies in this kit cross-react with IL-4 from non-human primates. See the Human/NHP cross-reactivity table.

Analyte Information

IL-4
Interleukin-4 (IL-4, IL4) is produced by activated T cells, especially differentiated CD4+ TH2-type cells, and stimulated mast cells and basophils. The most important effects of IL-4 are the regulation of helper T cell differentiation to the TH2 type and the regulation of IgE and IgG4 production by B cells.
Application:


ELISpot


Reactivity:


Human / NHP




Contents:


Biotinylated detection mAb (IL4-II)
Streptavidin-ALP
anti-CD3 mAb
Substrate (BCIP/NBT-plus)
2
Pre-coated white plates (mAb IL4-I)


Size:

2 plates

Other formats:

Click to view all Human/NHP IL-4products

Minimize workload with the
one-step
detection ELISpot
PRO
kit





I have compared
Mabtech
ELISpot assay to ELISpot assays we run at our company on regular basis "head-to-head" using identical samples.
Mabtech
's kits proved to be superior
to our protocol, had significantly lower background and provided better and higher detection of spots.








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Mabtech/Human IL-4 ELISpot
PLUS
(ALP)/3410-4APW-2/2 plates






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