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商品編號(hào)


3632-001-02



品牌


Trevigen



公司


Trevigen, Inc



公司分類(lèi)


Extracellular Matrix Proteins



Size

1 ml, 2 x 5 ml, 5 ml

商品信息

Description

Cultrex? Basement Membrane Extract (BME) is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. This extract provides a natural extracellular matrix hydrogel that polymerizes at 37°C to form a reconstituted basement membrane. Basement membranes are continuous sheets of specialized extracellular matrix that form an interface between endothelial, epithelial, muscle, or neuronal cells and their adjacent stroma and that play an essential role in tissue organization by influencing cell adhesion, migration, proliferation, and differentiation. The major components of BME include laminin, collagen IV, entactin, and heparan sulfate proteoglycans.

Cultrex? BME Type 3 is physiologically aligned with the
in vivo
environment and is recommended for xenografts, tumorgrafts, and other
in vivo
applications.

Quality control specifications:


Mouse colonies are routinely screened for pathogens using?mouse antibody production (MAP) testing.

Engelbreth-Holm-Swarm (EHS) tumor is LDEV-free.

PathClear? – tested negative by PCR for?17 bacterial and virus strains typically included in mouse antibody production (MAP) testing, plus 13 additional murine infectious agents including LDEV, for a total of 31 organisms and viruses.

No bacterial or fungal growth detected after incubation at 37°C for 14 days following
USP
sterility testing guidelines.

Endotoxin concentration ≤ 8 EU/ml by Limulus Amoebocyte Lysate (LAL) assay.

Protein concentration of 12 – 18 mg/ml.

No mycoplasma contamination detected by PCR.

Provided in Roswell Park Memorial Instituted (RPMI)?medium with 10 ?g/ml gentamycin.

Gel st
ABI
lity tested for a period of 14 days at 37°C.

BIOLOG
ical activity determined for each lot:


Tumor Growth Assay – BME Type 3 supports proliferation and growth of breast cancer cells (MCF7) embedded in the matrix for minimum of 8 days.









Catalog # 3632-010-02 includes :?



Catalog Number

Description

Qty



3632-005-02

Cultrex? Basement Membrane Extract, Type 3, PathClear?

2




Genetic Identification of SEMA3F as an Antilymphangiogenic Metastasis Suppressor Gene in Head and Neck Squamous Carcinoma
Colleen L. Do?i, Constantinos M. Mikelis, Michail S. Lionakis, Alfredo A. Molinolo, and J. Silvio Gutkind
Cancer Res., Jul 2015; 75: 2937 – 2948.
http://cancerres.aacrjournals.org/cgi/content/abstract/75/14/2937