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商品編號


CHI-HF-201FLT3L-C050



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

FLT3L; FMS-like Tyrosine Kinase 3 Ligand



Product Type

Protein



Properties



Source/Host

HEK 293 cells



Sequence

Human FLT3L (aa 27 -184) is fused at the C-terminus to a His-tag.



Crossreactivity

Human
Mouse



BIOLOG
ical Activity

The ED50 was determined by the dose-dependent stimulation of the proliferation of human AML5 cells is ≤ 1.0 ng/ml, corresponding to a specific activity of ≥ 1 x 10
6
units/mg.



MW

~25kDa (SDS-PAGE)



Purity

≥95% (SDS-PAGE)



Endotoxin Content

<0.01eu mu;g="" purified="" protein="" (lal="" test;="" lonza).="">0.01eu>



Reconstitution

Reconstitute 10?g vial with 100 ?l sterile water to a concentration of 0.1mg/ml.
Reconstitute 50?g vial with 100 ?l sterile water to a concentration of 0.5mg/ml.
Add 1X PBS to the desired protein concentration.



Formulation

Lyophilized from 0.2μm-filtered solution in PBS.



Other Product Data

NCBI reference AAI26294.1: FLT3L (human)



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Avoid freeze/thaw cycles.
PBS containing at least 0.1% BSA should be used for further dilutions.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.
Working aliquots are stable for up to 3 months when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





FMS-like tyrosine kinase 3 ligand (FLT3L) acts as a growth factor that increases the number of immune cells (lymphocytes (B cells and T cells)) by activating the hematopoietic
Progen
itors. It binds to FLT3 (CD135) which is found on multipotent
Progen
itor (MPP) and common lymphoid
Progen
itor (CLP) cells in mice. FLT3L induces the mobilization of the hematopoietic
Progen
itors and stem cells
in vivo
which may help the system to kill cancer cells. FLT3L is crucial for steady-state pDC (plasmacytoid dendritic cells) and cDC (classical dendritic cells) development. Deficiency of FLT3L causes a dramatic decrease in DC numbers, whereas increasing its avail
ABI
lity increase in DC numbers.