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商品編號


GWB-885624



品牌


GenWay



公司


GenWay



公司分類


Secondary Antibodies



Size

1 mg

商品信息

Description:
Antigen:
Dry Ice Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like cytochrome c and caspase-9, which are critical molecules in apoptosis, AIF localizes to mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis. These effects occur in both isolated nuclei and in the nuclei of live cells treated by microinjection and with apoptosis stimuli (2,3). AIF is highly conserved between human and mouse and is widely expressed (2). 1. Zamzami N, Kroemer G (1999) Condensed matter in cell death. Nature 401127-128.
Similarity:
Dry Ice Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like cytochrome c and caspase-9, which are critical molecules in apoptosis, AIF localizes to mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis. These effects occur in both isolated nuclei and in the nuclei of live cells treated by microinjection and with apoptosis stimuli (2,3). AIF is highly conserved between human and mouse and is widely expressed (2). 1. Zamzami N, Kroemer G (1999) Condensed matter in cell death. Nature 401127-128.
Buffer:
Dry Ice Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like cytochrome c and caspase-9, which are critical molecules in apoptosis, AIF localizes to mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis. These effects occur in both isolated nuclei and in the nuclei of live cells treated by microinjection and with apoptosis stimuli (2,3). AIF is highly conserved between human and mouse and is widely expressed (2). 1. Zamzami N, Kroemer G (1999) Condensed matter in cell death. Nature 401127-128.
Fraction:
Dry Ice Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like cytochrome c and caspase-9, which are critical molecules in apoptosis, AIF localizes to mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis. These effects occur in both isolated nuclei and in the nuclei of live cells treated by microinjection and with apoptosis stimuli (2,3). AIF is highly conserved between human and mouse and is widely expressed (2). 1. Zamzami N, Kroemer G (1999) Condensed matter in cell death. Nature 401127-128.
Function:
Dry Ice Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins (1). A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF) (2). Like cytochrome c and caspase-9, which are critical molecules in apoptosis, AIF localizes to mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis. These effects occur in both isolated nuclei and in the nuclei of live cells treated by microinjection and with apoptosis stimuli (2,3). AIF is highly conserved between human and mouse and is widely expressed (2). 1. Zamzami N, Kroemer G (1999) Condensed matter in cell death. Nature 401127-128.
Additional Information:




Name

Peroxidase Conjugated Affinity Purified Anti-MOUSE IgA (alpha chain) (RABBIT)



Related Product Names

Peroxidase Conjugated Affinity Purified anti-Mouse IgA (? heavy chain specific) [Rabbit]; N/A Peroxidase Conjugated Affinity Purified Anti-MOUSE IgA (alpha chain) (RABBIT)IGHA1



Alias Symbols

HRP



Clone

Polyclonal



Immunogen

Mouse IgA heavy chain



Product Format

Lyophilized



Molecular Weight

40000



Swiss Prot Number

P01876



Applications

WB,
ELISA
, IHC



Application Info

ELISA
: 1:200,000 WB: 1:2,000 - 1:10,000 IHC: 1:1,000 - 1:5,000



Format

Lyophilized



Reconstitution and Storage

Store vial at 4 C prior to restoration. ? ? Restore with 1.0 mL of deionized water (or equivalent). For extended storage aliquot contents and freeze at -20 C or below. ? Avoid cycles of freezing and thawing. ? Centrifuge product if not completely clear after standing at room temperature. ? This product is stable for several weeks at 4 C as an undiluted liquid. ? Dilute only prior to immediate use. ? ? Expiration date is one (1) year from date of restoration.



Reactivity

Mouse IgA (alpha chain)



Storage

Store vial at 4C prior to restoration. Restore with 1.0ml of deionized water (or equivalent). For extended storage mix product with glycerol to 50% and aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product



Lead Time

Domestic: within 1-2 weeks delivery?International: 1-2 weeks



Intended Use

Research Use Only



Additional Information

Buffer:
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Color:
Substrate dependent
Conjugation Reference:
Modified from Farr & Nakane, J. Immunol. Methods 47; 129-144. 1981.



::

Label:
Peroxidase (Horser
ADI
sh)
Preservative:
0.01% (w/v) Thimerisol
St
ABI
lizer:
10 mg/ml Bovine Serum Albumin (BSA) ?- Immunoglobulin and Protease free