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商品編號


GWB-147E17



品牌


GenWay



公司


GenWay



公司分類


Secondary Antibodies



Size

1 ml

商品信息

Additional Information:




Name

Rabbit Anti-Rat IgG (H+L) Absorbed against human immunoglobulins



Related Product Names

Rabbit Anti-Rat IgG(H+L), Human ads-AP; N/A; N/A Rabbit Anti-Rat IgG (H+L) Absorbed against human immunoglobulins



Gene Symbol

IGHG1



Gene Fullname

Ig gamma-1 chain C region



Protein Name

Ig gamma-1 chain C region



Swissprot ID

P01857



Host

Pooled antisera from rabbits hyperimmunized with rat IgG.



Isotype

IgG



Replacement

This antibody may replace item sc-52003 from Santa Cruz Biotechnology.



NCBI Acc Number

AAA51107.1



Product Format

Alkaline Phosphatase (AP) Conjugate



Purification

Affinity chromatography on pooled rat IgG covalently linked to agarose.



Specificity

Reacts with the heavy and light chains of rat IgG1, IgG2a, IgG2b and IgG2c, andwith the light chains of rat IgM as demonstrated by
ELISA
and/or flow cytometry.Minimal cross reactivity with human immunoglobulins.



Applications

IF,
ELISA
, WB, DB, IHC



Application Info

- Indirect immunofluorescent staining in conjunction with rat monoclonal antibodies
- Enzyme-Linked-Immunosorbent-Assay (
ELISA
)
- Western blotting
- Dot-and slot-immunoblotting
- Immunohistochemistry



GI Number

554058



Dilution

Immunofluorescence:
ELISA
:
FITC conjugate
BIOT conjugate
AP conjugate
HRP conjugate
BIOT conjugate
<= 1="">=>
6
cells
<= 1="">=>
6
cells
1:2,000-1:4,000
1:4,000-1:8,000
1:5,000-1:20,000



Clonality

Polyclonal



Format

Alkaline Phosphatase (AP) Conjugate



Absorption

Pooled human sera and purified human paraproteins.



Reactivity

Rat



Sequence Length

124



Warning

Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.



Storage

The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8C.
The fluorescein (FITC) conjugate is supplied as 1.0 m



Protocol

Reconstitution Instructions
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme- Linked ImmunoSorbent Assay (
ELISA
) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol



Lead Time

Domestic: within 1-2 weeks delivery?International: 1-2 weeks



Intended Use

Research Use Only