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商品編號


AG-40T-0350-C250



品牌


Adipogen



公司


Adipogen



公司分類


Proteins



Size

250 ?g

商品信息


More Information



Product Details



Synonyms

Receptor Associated Protein 80; RXRIP110; UIMC1; X2HRIP110; BRCA1-A Complex Subunit RAP80; Retinoid X Receptor-interacting Protein 110; Ubiquitin Interaction Motif-containing Protein 1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human RAP80 (UIM domains aa 79-124) (Accession Nr. NP_057374) fused to a His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES, 200mM NaCl, 1mM DTT pH 8.0.



Other Product Data


Use:
Can be used for the isolation and identification of ubiquitinated proteins that are non-K48-linked. Allows for metal chelate affinity purification and also allows for convenient immuno-detection of conjugates using His-specific antibodies. Use 50-100?g of protein to detect 10-20?g of purified K63-linked ubiquitin chains. The amount necessary for use in crude lysates needs to be determined empirically.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Rap80 (Receptor Associated Protein 80) interacts with BRCA1, a ubiquitin E3-ligase which functions in conjugation with the BARD1 deubiquitinating enzyme. BRCA1 is recruited to DNA damage sites by poly-ubiquitin chains through Rap80 which contains 2 tandem ubiquitin-interacting motifs (UIMs). Rap80 constitutes a protein complex with ABRA1 which interacts with the BRCT domain of BRCA1. Upon DNA damage the Rap80-ABRA1 complex targets the BRCA1-BARD1 complex to K6- and K63-linked poly-ubiquitin chains at these foci. The UIM domains of Rap80 have been shown to have preferential binding to K-6 and K63-linked ubiquitin chains and binds to K48-chain with a much lower efficiency. It is not known if Rap80 UIMs interact with ubiquitin chains linked via K11, K27 or K33. Rap80 has a low affinity for mono-, di- and tri-ubiquitin but binds efficiently to tetra (or greater) ubiquitin chains.