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商品編號(hào)


GWB-1F8C09



品牌


GenWay



公司


GenWay



公司分類


Secondary Antibodies



Size

0.25 mg

商品信息

Additional Information:




Name

Goat F(ab')2 Anti-Rat IgG (H+L chain specific) Absorbed against mouse immunoglobulins



Related Product Names

Goat F(ab')2 Anti-Rat IgG(H+L), Mouse ads-PE/CY5.5; N/A Goat F(ab')2 Anti-Rat IgG (H+L chain specific) Absorbed against mouse immunoglobulinsIGHG1



Gene Symbol

IGHG1



Gene Fullname

Ig gamma-1 chain C region



Protein Name

Ig gamma-1 chain C region



Swissprot ID

P01857



Host

Pepsin digest of goat anti- rat IgG (H+L).



Isotype

IgG



Replacement

This antibody may replace item sc-52003 from Santa Cruz Biotechnology.



Product Format

R- phycoerythrin- Cyanine 5.5 (R- PE- CY5.5) Conjugate



Swiss Prot Number

P01857



Purification

Gel filtration of pepsin- digested antibody.



Specificity

Reacts with the heavy and light chains of rat IgG, and with the light chains of ratIgM and IgA as demonstrated by
ELISA
, FLISA, and flow cytometry. Minimalcross reactivity with mouse immunoglobulins.



Applications

Direct and IF,
ELISA
, IB, IHC



Application Info

- Direct and indirect immunofluorescent staining
- Enzyme-Linked-Immunosorbent-Assay (
ELISA
)
- Fluorescent-Linked-Immunosorbent-Assay (FLISA)
- Immunoblotting
- Immunohistochemistry



Dilution

Immunofluorescence:
FLISA:
ELISA
:
UNLB, FITC, and BIOT conjugates
R-PE, R-PE-TXRD, SPRD and R-PE-CY?5.5
conjugates
TXRD conjugates
BIOT conjugate
<= 1="">=>
6
cells
<= 0.1="">=>
6
cells
1:100 – 1:400
1:5,000-1:20,000



Clonality

Polyclonal



Format

**R-phycoerythrin-Cyanine 5.5 (R-PE-CY?5.5) Conjugate



Absorption

Pooled mouse sera and pooled mouse plasmacytoma/hybridoma proteins.



Reactivity

Rat



Warning

Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.



Storage

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8C.
The fluorescein (FITC) and Texas Red? (TXRD) conjugat



Protocol

Reconstitution Instructions
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme- Linked ImmunoSorbent Assay (
ELISA
) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol



Lead Time

Domestic: within 1-2 weeks delivery?International: 1-2 weeks



Intended Use

Research Use Only