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商品編號(hào)


GWB-3B07EF



品牌


GenWay



公司


GenWay



公司分類


Secondary Antibodies



Size

1 ml

商品信息

Additional Information:




Name

Rabbit F(ab')2 Anti-Mouse IgG (H+L)



Related Product Names

Rabbit F(ab')2 Anti-Mouse IgG(H+L)-HRP; N/A Rabbit F(ab')2 Anti-Mouse IgG (H+L)IGHG1



Gene Symbol

IGHG1



Gene Fullname

Ig gamma-1 chain C region



Protein Name

Ig gamma-1 chain C region



Swissprot ID

P01857



Host

Pepsin digest of rabbit anti- mouse IgG (H+L).



Isotype

IgG



Replacement

This antibody may replace item sc-52003 from Santa Cruz Biotechnology.



Product Format

Horser
ADI
sh Peroxidase (HRP) Conjugate



Swiss Prot Number

P01857



Purification

Affinity chromatography on mouse IgG covalently coupled to agarose. Gelfiltration chromatography of pepsin digested antibody.



Specificity

Reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b and IgG3, andwith the light chains of mouse IgM and IgA as demonstrated by
ELISA
and flowcytometry.



Applications

IF,
ELISA
, WB, DB, IHC



Application Info

- Direct immunofluorescent staining
- Enzyme-Linked-Immunosorbent-Assay (
ELISA
)
- Western blotting
- Dot-and slot-immunoblotting
- Immunohistochemistry



Dilution

Immunofluorescence:
ELISA
:
FITC
AP conjugate
HRP conjugate
<= 1="">=>
6
cells
1:2,000-1:4,000
1:4,000-1:8,000



Clonality

Polyclonal



Format

Horser
ADI
sh Peroxidase (HRP) Conjugate



Absorption

NA



Reactivity

Mouse



Warning

Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.



Storage

The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8C.
The fluorescein (FITC) conjugate is supplied as 0.5 m



Protocol

Reconstitution Instructions
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme- Linked ImmunoSorbent Assay (
ELISA
) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol



Lead Time

Domestic: within 1-2 weeks delivery?International: 1-2 weeks



Intended Use

Research Use Only