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商品編號(hào)


AG-40T-0394-C100



品牌


Adipogen



公司


Adipogen



公司分類


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Four human ubiquitin L73P mutants (Accession Nr. P0CG47) covalently linked through the N-terminal amino group of methionine on one ubiquitin that is conjugated with the C-terminal carboxy group of another ubiquitin.



Crossreactivity

Human



Formulation

Lyophilized from a solution in deionized water.



Other Product Data


Use:
This linear ubiquitin fusion protein is resistant to the activity of deubiquitinating enzymes (DUB's) that cleave the peptide linkage between adjacent ubiquitin molecules. Useful for investigating ubiquitin-binding proteins and exploring the role of unanchored (free C-terminus) ubiquitin chains in signaling pathways. Reaction conditions will need to be optimized for each specific application.
IMPORTANT:
Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40°c for="" 20="" minutes="" prior="" to="" gel="" electrophoresis.="">40°c>



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





With a predicted molecular weight of 34 kDa, Tetra-ubiquitin chains are composed of four ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin molecule. Each human ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa identity with yeast and mouse ubiquitin, respectively. Seven of the 76 aa in ubiquitin are lysine residues that can participate in poly-ubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking, and other cellular processes. Ubiquitin is not expressed directly as free ubiquitin, but rather as linear fusions either to itself or to certain ribosomal protein subunits. These ubiquitin-fusion precursors are proteolyzed by DUB's at the appropriate junction points to yield active ubiquitin monomers with C-termini ending in GG. There are likely several intracellular DUB's which perform this essential processing role.