GenWay/Mouse IgG1 Antibody (HRP)/GWB-5419A3/1 ml
二抗
商品編號
GWB-5419A3
品牌
GenWay
公司
GenWay
公司分類
Secondary Antibodies
Size
1 ml
商品信息
Description:
Short Description:
GOAT ANTI MOUSE IgG1HRP
Additional Information:
Name
Mouse IgG1 Antibody (HRP)
Related Product Names
GOAT ANTI MOUSE IgG1:HRP
Gene Symbol
Ighg1
Gene Family
IGH
Gene id
16017
Alias Symbols
IgG1, Igh-4, VH7183, Ighg1
Protein Name
Ig gamma-1 chain C region, membrane-bound form
Description of Target
GOAT ANTI MOUSE IgG1:HRP
Swissprot ID
P01869
Species Reactivity
Mouse
Host
Goat
Isotype
Polyclonal IgG
Conjugation
HRP
Replacement
This antibody may replace item sc-52003 from Santa Cruz Biotechnology.
Immunogen
Mouse IgG1 paraproteins
Homology
Mouse
Product Format
Purified IgG conjugated to Horser
ADI
sh Peroxidase (HRP) - liquid
Swiss Prot Number
P01869
Specificity
IgG1
Applications
C, E, P, WB
Application Info
ELISA
:
? ?1/4000 - 1/8000
Clonality
Polyclonal
Format
Solution: Phosphate buffered saline, Stabalizer: 0.01% Thiomersal , 50% Glycerol, Form: Purified IgG conjugated to Horser
ADI
sh Peroxidase (HRP) - liquid
St
ABI
lity
18 months from date of despatch.
Reconstitution and Storage
Store at +4
o
C or at -20
o
C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Reactivity
Mouse
Storage
Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitat
Protocol Information
Citation:
1: Andersen P. Effective vaccination of mice against Mycobacterium tuberculosisinfection with a soluble mixture of secreted mycobacterial proteins. Infect Immun. 1994 Jun;62(6):2536-44. PubMed PMID: 7910595; PubMed Central PMCID: PMC186542.
Species:
Mouse, Bacteria
Experiment Name:
Antibody titers to ST-CF of different IgG isotypes.
Experiment Background:
The relative levels of specific IgGl and IgG2A induced by the different doses of ST-CF were used to give an indirect indication of the lymphocyte subsets involved.
Experimental Steps:
1. Serum samples obtained from the mice 5 weeks after the first immunization were analyzed in two fold dilutions on
ELISA
platescoated with ST-CF (100 ?l per well; 5 ,ug/ml). 2. The plates wereincubated for 2 h at room temperature, and, after washing, theplates received peroxidase-labeled goat antimouse immunoglobulinG (IgG), IgG2A (1/1,000), or IgGl (1/2,000) (Kidlington, Oxford, United Kingdom). 3. Antibody titers toST-CF of different isotypes are expressed as the dilutions thatgave an absorbency of 1 (IgGl) or 0.4 (IgG2A).4. The titers havebeen adjusted for different levels of specific IgG induced by thevaccines and are therefore presented as relative isotype titers.
Other Reagents Used:
DDA, phosphatidylcholindistearoyl, TDM
Number Of Protocols:
1
Lead Time
Domestic: within 2-3 weeks delivery?International: 2-3 weeks
Intended Use
Research Use Only
::
Preservative St
ABI
lisers:
0.01% Thiomersal
50% Glycerol
Antiserum Preparation:
Antisera to murine IgG1 were raised by repeated immunisation of goats with purified antigen. Purified IgG was prepared by affinity chromatography.
::
Buffer Solutions:
Phosphate buffered saline pH7.4
產(chǎn)品貨號:3853.6