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商品編號(hào)


GWB-545470



品牌


GenWay



公司


GenWay



公司分類


Secondary Antibodies



Size

0.5 mg

商品信息

Description:
Short Description:
GOAT ANTI MOUSE IgGHRP (RAT ADSORBED)
Additional Information:




Name

Mouse IgG Antibody (HRP) (rat adsorbed)



Related Product Names

GOAT ANTI MOUSE IgG: HRP (RAT ADSORBED) IgGIGHG1



Gene Symbol

Ighg1



Gene Family

IGH



Gene id

16017



Alias Symbols

IgG1, Igh-4, VH7183, Ighg1



Protein Name

Ig gamma-1 chain C region, membrane-bound form



Description of Target

GOAT ANTI MOUSE IgG:HRP (RAT ADSORBED)



Swissprot ID

P01869



Species Reactivity

Mouse



Host

Goat



Isotype

Polyclonal IgG



Conjugation

HRP



Replacement

This antibody may replace item sc-52003 from Santa Cruz Biotechnology.



Immunogen

Mouse IgG



Homology

Mouse



Product Format

Purified IgG conjugated to Horser
ADI
sh Peroxidase (HRP) - liquid



Swiss Prot Number

P01869



Specificity

IgG



Concentration

0.5mg/ml



Applications

C, E, P



Application Info

Immunohistology - Frozen:
? ?1/50
ELISA
:
? ?1/500 - 1/1000



Clonality

Polyclonal



Format

Solution: Phosphate buffered saline, Stabalizer: 0.01% Thiomersal, HRP St
ABI
liser , Form: Purified IgG conjugated to Horser
ADI
sh Peroxidase (HRP) - liquid



St
ABI
lity

18 months from date of despatch.



Reconstitution and Storage

Store at +4
o
C. DO NOT FREEZE.
This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.



Reactivity

Mouse



Storage

Store at +4°C. DO NOT FREEZE. This product should be stored undiluted. Should this product contain a precipitate we recommend microcentrifugation before use.



Protocol Information

Citation:
1: Dennis A, Kudo T, Kruidenier L, Girard F, Crepin VF, MacDonald TT, Frankel G, Wiles S. The p50 subunit of NF-kappaB is critical for in vivo clearance of thenoninvasive enteric pathogen Citrobacter rodentium. Infect Immun. 2008Nov;76(11):4978-88. Epub 2008 Aug 11. PubMed PMID: 18694964; PubMed CentralPMCID: PMC2573329.
Species:
The bacterial strain Citrobacter rodentium, Mouse
Experiment Name:
1. Immunohistochemistry and 2. Determination of C. rodentium-specific antibody responses.
Experiment Background:
1. In this work, Alison et al. characterized the immune response and pathology of mice lacking the p50 subunit of the transcription factor nuclear factor kappa B (NF-?B) during C. rodentiuminfection. They showed that p50–/– mice are unable to clear C. rodentium infection. 2. Mice lacking p50 show delayed and reduced immune cell infiltration into colonic tissue during C. rodentium infection. Immune cell infiltrates were measured during C. rodentium infection of p50+/+ mice and p50–/– mice using frozen sections of murine colon incubated with antibodies against CD3 , CD4, CD8, CD11c as a
Marker
for dendritic cells [DC], and F4/80 as a
Marker
for macrophages [M?]
Experimental Steps:
1 Immunohistochemistry: Cryostat sections of murine colon were rehydrated in Tris-buffered saline (TBS) for 5 min and then incubated with antibodies against CD3, CD4, CD8, F4/80, CD11c, NK1.1, or CD45R (Oxford, United Kingdom) at a 1:10 to 1:100 dilution for 1 h. The sections were gently washed three times with TBS before the addition of biotinylated anti-rat or anti-hamster immunoglobulin G (IgG) , as appropriate, at a dilution of 1:200 with 4% (vol/vol) normal murine serum for blocking (Sera Laboratories International,Horsted Keynes, United Kingdom) for 30 min. After the washing, a 1:200 dilution of 0.1% avidin-peroxidase (
Sigma-Aldrich
) was added for 30 min before further washing and the addition of diaminobenz
ADI
ne substrate (
Sigma-Aldrich
) for 5 to 10 min. The reaction was stopped with excess TBS, and the sections were counterstained with hematoxylin and mounted. A control slide using no primary antibody was also used to show endogenous peroxidase-containing cells. Stained cell populations were counted in five randomly selected fields per section, and the data were expressed as the number of T cells per 250 ?m2 of lamina propria.2 Determination of C. rodentium-specific antibody responses. An 18-h culture of C. rodentium was res
USP
ended in PBS plus 1% bacterial protease inhibitor (
Sigma-Aldrich
Ltd., Dorset, United Kingdom) to an optical density at 600 nm (OD600) of 1.0 and then heat killed at 60°C for 1 h. A 1:50 PBS dilution of this culture was added to
ELISA
plates (
BD
Biosciences, Oxford, United Kingdom) at 100 ?l per well before incubation at 4°C overnight. The plates were washed three times with 200 ?l per well PBS plus 0.05% Tween and then blocked for 1 h with 200 ?l per well 1% bovine serum albumin in PBS blocking buffer. At selected time points postinoculation, blood was collected from the mice by cardiac puncture and centrifuged at 8,000 x g to separate red blood cells from serum. The C. rodentium-coated
ELISA
plates were washed. and then serum samples diluted 1:100 with PBS were added in duplicate wells (50 ?l per well) and incubated at room temperature for 2 h. The plates were washed as before and then incubated at room temperature for 2 h with 100 ?l per well 1:2,000 blocking buffer dilutions of rat anti-mouse IgG, IgG2b, IgG3, or IgM conjugated to alkaline phosphatase (A
BD
, Oxford, United Kingdom). After the plates were washed, antibodies were detected with 100 ?l per well 1-mg/ml p-nitrophenylphosphate (
Sigma-Aldrich
Ltd., Dorset, United Kingdom) for 5 to 10 min before the reaction was stopped with 25 ?l per well 3 N NaOH. Absorbance was determined using a VersaMax microplate reader with SoftmaxPRO software (Molecular Devices,California) at 405 nm.
Other Reagents Used:
kanamycin , nalidixic acid
Number Of Protocols:
2



Lead Time

Domestic: within 2-3 weeks delivery?International: 2-3 weeks



Intended Use

Research Use Only



::

Preservative St
ABI
lisers:
0.01% Thiomersal
1%Bovine Serum Albumin
HRP St
ABI
liser (BUF052A)
Antiserum Preparation:
Antisera to mouse IgG were raised by repeated immunisation of goats with highly purified antigen. Purified IgG was prepared by affinity chromatography.



::

Approx Protein Conc:
IgG concentration 0.5 mg/ml
Buffer Solutions:
Phosphate buffered saline