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商品編號


AG-40T-0565-C025



品牌


Adipogen



公司


Adipogen



公司分類


Proteins



Size

25 ?g


商品信息


More Information



Product Details



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Three human ubiquitin (Accession Nr. P0CG47) covalently linked through isopeptide bonds at K6 residues of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin molecule.



Crossreactivity

Human



MW

~26kDa



Purity

≥95% (SDS-PAGE)



Concentration

1mg/ml



Formulation

Liquid. In sterile, deionized water.



Other Product Data


Use:
Formed with wild-type human recombinant ubiquitin and linkage-specific enzymes. Validated by Ub-AQUA, an LCMS-based technique that provides extremely accurate information on the composition of Poly-ubiquitin samples. Linkage-specific tri-ubiquitin can be used as a substrate for enzymes that cleave the isopeptide linkage between ubiquitin molecules. It can also be used to investigate mechanism of binding and recognition by E1 or E2 enzymes, deubiquitinating enzymes, E3 ligases or other proteins that contain ubiquitin-associated domains (UBAs) or ubiquitin-interacting motifs (UIMs). Ideal for investigating ubiquitin-binding proteins and as substrates for ubiquitin-specific isopeptidases. Reaction conditions will need to be optimized for each specific application.
IMPORTANT:
Heating this product in SDS-PAGE buffer or terminating reactions containing this product with heated SDS-PAGE buffer could lead to unexpected, high apparent molecular weight banding or smearing on gels that is not representative of product purity. For optimal results, we recommend incubation in SDS-PAGE buffer + DTT at <40°c for="" 20="" minutes="" prior="" to="" gel="" electrophoresis.="">40°c>



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


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With a predicted molecular weight of 26 kDa, tri-ubiquitin chains are composed of three ubiquitin monomers that are covalently linked through isopeptide bonds, which typically form between a lysine residue of one ubiquitin molecule and the C-terminal glycine residue of another ubiquitin. Each human ubiquitin monomer is 76 amino acids (aa) in length and shares 96% and 100% aa sequence identity with yeast and mouse ubiquitin, respectively. Seven of the 76 aa in ubiquitin are lysine residues that can participate in poly-ubiquitin chain formation. Linkage through specific lysine residues is thought to serve as a signal that affects protein degradation, signaling, trafficking and other cellular processes.