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商品編號


AG-40T-0406-C050



品牌


Adipogen



公司


Adipogen



公司分類


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms


USP
1-associated Factor 1; WD Repeat-containing Protein 48; WDR48



Product Type

Protein



Properties



Source/Host

Sf21 cells



Sequence

Human UAF1 (Accession Nr. Q8TAF3) fused to a C-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In HEPES, NaCl, TCEP and Glycerol.



Other Product Data


Use:
Activator of ubiquitin-specific deconjugating enzymes
USP
1,
USP
12, and
USP
46. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration equal to the concentration of deconjugating enzyme present (1:1 stoichiometry) in
in vitro
deconjugation reactions utilizing Ubiquitin-AMC.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





UAF1 (
USP
1-Associated Factor 1, also known as WD Repeat-containing protein 48 or WDR48) is a positive regulator of at least three deubiquitinating enzymes of the C19 peptidase family including
USP
1,
USP
12 and
USP
46. In the absence of UAF1, all three of these deubiquitinases are almost completely inactive and available biochemical evidence suggests UAF1 activation occurs by increasing catalytic turnover as opposed to altered substrate affinity (at least in the case of UAF1/
USP
1) of the enzymes. Most notably, UAF1 strongly activates
USP
1 deubiquitination of monoubiquitinated FANCD2 and PCNA, thereby playing important roles in DNA damage response and translesion synthesis. The UAF1/
USP
12 complex has recently been described as a negative regulator of Notch signaling. Disruption of UAF1-dependent
USP
12 activity interrupts Notch trafficking to the lysosomes, le
ADI
ng to an increased amount of receptor at the cell surface and to higher Notch activity. At the biochemical level, UAF1/
USP
12 deubiquitinates the non-activated form of Notch in cell culture and
in vitro
.