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商品編號(hào)


GWB-5EE4E0



品牌


GenWay



公司


GenWay



公司分類(lèi)


Secondary Antibodies



Size

1 mg

商品信息

Additional Information:




Name

Goat Anti-Rat IgG (H+L chain specific) Absorbed against mouse immunoglobulins



Related Product Names

Goat Anti-Rat IgG(H+L), Mouse ads-UNLB; N/A Goat Anti-Rat IgG (H+L chain specific) Absorbed against mouse immunoglobulinsIGHG1



Gene Symbol

IGHG1



Gene Fullname

Ig gamma-1 chain C region



Protein Name

Ig gamma-1 chain C region



Swissprot ID

P01857



Host

Pooled antisera from goats hyperimmunized with normal rat IgG.



Isotype

IgG



Replacement

This antibody may replace item sc-52003 from Santa Cruz Biotechnology.



Product Format

Purified (UNLB) Antibody



Swiss Prot Number

P01857



Purification

Affinity chromatography on pooled IgG covalently linked to agarose.



Specificity

Reacts with the heavy and light chains of rat IgG, and with the light chains of ratIgM as demonstrated by
ELISA
, FLISA, and/or flow cytometry. Minimal crossreactivity with mouse immunoglobulins.



Applications

Direct and IF,
ELISA
, IB, IHC



Application Info

- Direct and indirect immunofluorescent staining
- Enzyme-Linked-Immunosorbent-Assay (
ELISA
)
- Fluorescent-Linked-Immunosorbent-Assay (FLISA)
- Immunoblotting
- Immunohistochemistry



Dilution

Immunofluorescence:
FLISA:
ELISA
:
FITC and BIOT conjugates
R-PE conjugate
TRITC and TXRD conjugates
AP conjugate
HRP conjugate
BGAL conjugate
BIOT conjugate
<= 1="">=>
6
cells
<= 0.1="">=>
6
cells
1:200-1:400
1:2,000-1:4,000
1:4,000-1:8,000
1:500
1:5,000-1:20,000



Clonality

Polyclonal



Format

Purified (UNLB) Antibody



Absorption

Pooled mouse sera and pooled mouse plasmacytoma/hybridoma proteins.



Reactivity

Rat



Warning

Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes,or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copiousamounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoicacid under acidic conditions. Dilute azide- containing compounds in running water before discarding to avoidaccumulation of potentially explosive deposits in lead or copper plumbing.



Storage

The purified (UNLB) antibody is supplied as 1.0 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2-8C.
The fluorescein (FITC), rhodamine (TRITC), and Texas



Protocol

Reconstitution Instructions
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme- Linked ImmunoSorbent Assay (
ELISA
) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol



Lead Time

Domestic: within 1-2 weeks delivery?International: 1-2 weeks



Intended Use

Research Use Only