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商品編號


AG-40T-0431-C050



品牌


Adipogen



公司


Adipogen



公司分類


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin E1 Enzyme; Ubiquitin Activating Enzyme E1; Ubiquitin-like Modifier-activating Enzyme 1; A1S9T; AMCX1; GXP1; POC20; SMAX2; UBA1



Product Type

Protein



Properties



Source/Host

Sf21 cells



Sequence

Human UBE1 (Accession Nr. P22314) fused to a N-terminal His-tag.



Crossreactivity

Human



MW

~121kDa



Purity

≥95% (SDS-PAGE)



Formulation

Liquid. In 50mM HEPES pH 8.0, 50mM NaCl, 1mM TCEP.



Other Product Data


Use:
Member of the ubiquitin-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a ubiquitin-conjugating (E2) enzyme and a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 50-200nM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin-activating Enzyme (UBE1), also known as ubiquitin-like modifier activating enzyme 1 (UBA1), is a 1058 amino acid (aa) canonical member of the ubiquitin-activating (E1) enzyme family of proteins with a predicted molecular weight of 118 kDa. It is ubiquitously expressed and highly conserved; mouse and rat UBE1 share 95% and 96% aa sequence identity with the human UBE1 protein, respectively. UBE1 is found in the cytoplasm and nucleus, and contains a conserved active-site cysteine residue and ATP-binding site common to E1 enzymes. UBE1 is respons
IBL
e for the first step in ubiquitin-protein isopeptide bond formation. Ubiquitin is activated by UBE1 and thereafter linked to the side chain of a cysteine residue in UBE1, Cys632 in humans, yielding a ubiquitin-UBE1 conjugate via a thioester bond. The activated ubiquitin is then transferred to a lysine residue on the target protein via the ubiquitin-conjugating – ubiquitin ligase enzyme cascade. UBE1 is required for cell cycle progression and has been linked to cellular responses to DNA damage such as nucleotide excision repair. Mutations in UBE1 are associated with X-linked lethal infantile spinal muscular atrophy. UBE1 is a critical component for the initiation of
in vitro
ubiquitin conjugation reactions.