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商品編號(hào)


GWB-6094FA



品牌


GenWay



公司


GenWay



公司分類


Secondary Antibodies



Size

0.5 mg

商品信息

Description:
Short Description:
GOAT ANTI RAT IgG2aFITC
Additional Information:




Name

Rat IgG2a Antibody (FITC)



Related Product Names

GOAT ANTI RAT IgG2a: FITC IgG2aIgg-2a



Gene Symbol

LOC679045



Gene id

314494



Protein Name

Ig gamma-2A chain C region



Description of Target

GOAT ANTI RAT IgG2a:FITC



Swissprot ID

P20760



Species Reactivity

Rat



Host

Goat



Isotype

Polyclonal IgG



Conjugation

FITC



Homology

Rat



Product Format

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid



Swiss Prot Number

P20760



Specificity

IgG2a



Concentration

1mg/ml



Applications

F



Application Info

Flow Cytometry:
? ?1/500



Clonality

Polyclonal



Format

Solution: Phosphate buffered saline, Stabalizer: 0.09%, Sodium Azide. 0.2%, Bovine Serum Albumin, Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid



St
ABI
lity

18 months from date of despatch.



Reconstitution and Storage

Store at +4
o
C or at -20
o
C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.



Reactivity

Rat



Storage

Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this m



Protocol Information

Citation:
1: López MC, Holmes N. Phenotypical and functional alterations in the mucosalimmune system of CD45 exon 9 KO mice. Int Immunol. 2005 Jan;17(1):15-25. Epub2004 Nov 22. PubMed PMID: 15557316.
Species:
Mouse
Experiment Name:
1. Flow cytometric staining2. Immunomicroscopy of Peyer’s patch and mesenteric lymph node cells
Experiment Background:
Exon 9 CD45 KO mice possess a complete CD45 null phenotype in thymus, spleen and lymph nodes.It was established by using both flow cytometry and tissue staining that CD45 is absent from intestinal intraepithelium and lamina propria lymphocytes. Cells recovered from CD45 KO PP (Peyer’s patch) and MLN (mesenteric lymph node) were larger than cells from normal mice
Experimental Steps:
1. Flow cytometric staininga) Small intestine IELs from CD45 KO and normal mice were stained with rat anti-mouse CD45 (clone YBM 42.2) followed by a FITC-conjugated mouse anti-rat IgG2a showing a completely null phenotype.b) Small iIELs were isolated and stained with directly conjugated antibodies before being analyzed in a FACScan.2. Immunomicroscopy of Peyer’s patch and mesenteric lymph node cells Cytospins were prepared using PP and MLN cells in a Shandon cytocentrifuge (
Thermo
Shandon, Pittsburgh, PA). They were air-dried, fixed in methanol, air-dried and stored at -80°C until stained. After staining Cytospins were incubated for in the presence of biotinanti-mouse IgM and FITC-anti-mouse IgA (Southern Biotechnology). Cytospins were washed and sequentially incubated with streptavidin, followed by biotinylated-goat anti-streptavidin (both Vector, Burlingame, CA) and Texas Red streptavidin (
Jackson
ImmunoResearch), all incubations lasted for 30 min and washes were performed in between incubations and slides were analyzed.
Number Of Protocols:
2



Lead Time

Domestic: within 2-3 weeks delivery?International: 2-3 weeks



Intended Use

Research Use Only



::

Preservative St
ABI
lisers:
0.09% - Sodium Azide
0.2% - Bovine Serum Albumin
Antiserum Preparation:
Antisera to rat IgG2a were raised by repeated immunisations of goats with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.



::

Approx Protein Conc:
IgG concentration 1.0 mg/ml
Buffer Solutions:
Phosphate buffered saline pH7.2