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商品編號


SBB-CE0019-C100



品牌


Adipogen



公司


Adipogen



公司分類


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme H5c; PUBC; UBC5C; Ubiquitin-protein Ligase D3; p53-regulated Ubiquitin-conjugating Enzyme



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UBE2D3 (aa1-147) (Accession Nr. P61077) is untagged.



Crossreactivity

Human



Application

Typical working concentration range is 1-5?M.



MW

~17kDa



Purity

≥95% (SDS-PAGE)



Concentration

Lot dependent.



Accession Number

P61077



Formulation

Liquid. In 50mM HEPES pH 8, 200mM sodium chloride, 10% glycerol (v/v), 1mM TCEP.



Other Product Data

Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.



Declaration

Manufactured by South Bay Bio.



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-80°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





UBE2D3 [UbcH5c] is an E2 ubiquitin conjugating enzyme. An E1 activating enzyme is required to attach ubiquitin to UBE2D3 via an active site cysteine. The mechanism of ubiquitin transfer involves the breaking of a E1-Ub thioester linkage, followed by a reformation of a UBE2D3-Ub thioester. UBE2D3 is capable of associating with numerous known E3 ligases including MDM2, which target proteins such as p53 for proteasomal degradation through polyubiquitination.