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商品編號(hào)


AG-40T-0433-C100



品牌


Adipogen



公司


Adipogen



公司分類


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme E2 D4; HBUCE1; Ubch5d; Ubiquitin-protein Ligase D4



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UBE2D4 (Accession Nr. Q9Y2X8) fused to a N-terminal His-tag.



Crossreactivity

Human



MW

17.5kDa



Purity

≥95% (SDS-PAGE)



Formulation

Liquid. In 50mM HEPES pH 8.0, 200mM NaCl, 10% Glycerol (v/v), 1mM TCEP.



Other Product Data


Use:
Member of the ubiquitin?conjugating (E2) enzyme family that receives ubiquitin from a ubiquitin?activating (E1) enzyme and subsequently interacts with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1?-1μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin-conjugating enzyme E2D 4 (UBE2D4), also known as ubiquitin-conjugating enzyme H5d (Ubch5d), is a member of the ubiquitin-conjugating (E2) enzyme family. Human UBE2D4/Ubch5d is 147 amino acids in length and has a predicted molecular weight of approximately 16.5 kDa. It may function with the IDOL ubiquitin ligase (E3) to ubiquitinate LDL-R. UBE2D4/Ubch5d, along with other UBE2D family members, may also be involved in the degradation of p53. Cadmium exposure has been reported to reduce the mRNA expression level of UBE2D4/Ubch5d.