欧美自拍另类欧美宗合图片区,国产视频一区二区三区四区,日本一区二区三区四区视频,婬片一区AAA毛片一区二区

商品編號


AG-40T-0434-C100



品牌


Adipogen



公司


Adipogen



公司分類


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-conjugating Enzyme E2 E3; E2-23K; UBCE4; UbcH9; UbcM2; Ubiquitin-protein Ligase E3



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human UBE2E3 (Accession Nr. NP_006348.1) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 8.0, 200mM NaCl, 10% Glycerol (v/v), 1mM TCEP.



Other Product Data


Use:
Member of the ubiquitin?conjugating (E2) enzyme family that receives ubiquitin from a ubiquitin?activating (E1) enzyme and subsequently interacts with a ubiquitin ligase (E3) to conjugate ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-?1μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin-conjugating Enzyme E2E 3 (UBE2E3), also known as ubiquitin-conjugating enzyme H9 (UbcH9), is a member of the ubiquitin-conjugating (E2) enzyme family. UBE2E3 has a predicted molecular weight of 23 kDa. The human protein shares 100% amino acid sequence identity with the mouse and rat orthologs. UBE2E3 has an E2 catalytic core domain with an active site cysteine residue that is required for the formation of a thioester bond with ubiquitin. This enzyme appears to be localized in the nucleus of interphase cells and cell-wide in mitotic cells. UBE2E3 may be regulated at the post-transcriptional level by miR-143. The auto-ubiquitination of the ARA54, RNF8, and bacterially-encoded NleG ubiquitin ligases (E3s) is mediated by UBE2E3. UBE2E3 also functions with NEDD4L to regulate ENaC activity. Physiologically, UBE2E3 is required for the proliferation of retinal pigment epithelial cells via the negative regulation of p27/Kip1 levels. UBE2E3 may also be required for HIV-1 replication.