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商品編號(hào)


AG-40T-0451-R250



品牌


Adipogen



公司


Adipogen



公司分類


Proteins



Size

250 ?l

商品信息


More Information



Product Details



Synonyms

Protein Linking IAP with
Cytoskeleton
1; DA41; DSK2; PLIC-1; UBQLN1; UBQN; XDRP1



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human ubiquilin 1 (TUBE2 aa 546-586) (Accession Nr. Q9UMX0) is conjugated to agarose.



Crossreactivity

Human



Label/Conjugates

Agarose



Formulation

Liquid. In 50mM HEPES, pH 7.5, 250mM NaCl.



Other Product Data


Use:
This affinity resin can be used for the enrichment, isolation and identification of K48-linked (preferentially) or K63-linked poly-ubiquitin chains or ubiquitinated substrates that contain these linkages. Equilibrate resin by washing with 5-10ml desired start buffer. Binding and elution of material is dependent on individual experimental conditions.

Storage:
The agarose can be re-used for at least 2 applications if properly maintained. After use, clean resin with 5ml 50mM Tris pH 9.0, 1M KCl. Remove cleaning solution by washing resin with 5ml storage buffer.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

+4°C



Handling Advice

Do not freeze.



Use/St
ABI
lity

Stable for at least 3 months after receipt when stored at +4°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquilin 1 is a ubiquitously expressed member of the UBL-UBA family of ubiquitin-binding proteins that is 589 amino acids (aa) in length and has a predicted molecular weight of 62.5 kDa. Human Ubiquilin 1 shares 88% aa sequence identity with the mouse and rat orthologs. It contains a N-terminal ubiquitin-like (UBL) domain and a C-terminal ubiquitin-associated (UBA) domain. The UBA domain is able to interact with mono- and poly-ubiquitin chains, with a similar affinity for Lys48- and Lys63-linked chains. In contrast, the UBL domain interacts with ubiquitin-interacting motif (UIM)-containing proteins, such as S5a/Angiocidin, a 19S Proteasome component. It is thought that Ubiquilin 1 may act as a molecular shuttle, bringing poly-ubiquitinated proteins to the 26S Proteasome by simultaneously binding poly-ubiquitin via its UBA domain and the 19S Proteasome via its UBL domain. Ubiquilin 1 also binds two endoplasmic reticulum (ER)-localized proteins, Herp and Erasin, and may function in the ER-associated degradation pathway. It has been reported to co-precipitate with LC3/MAP1LC3A/Apg8p3, an autophagosomal
Marker
, suggesting that it may play a role in autophagy. Single nucleotide polymorphisms in the UBQLN1 gene have been linked to Alzheimer's disease (AD). Fur
Thermo
re, Ubiquilin 1 may regulate the processing of APP, which is associated with AD.Tandem ubiquitin Binding Entities (TUBEs) have been developed for the isolation and identification of ubiquitinated proteins. TUBEs display increased affinity for poly-ubiquitin moieties over the single ubiquitin binding associated domain (UBA). TUBEs also display a protective effect on poly-ubiquitinated proteins, allowing for detection at relatively low abundance.