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商品編號(hào)


SBB-PS0001-C050



品牌


Adipogen



公司


Adipogen



公司分類


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

UBB; Ubiquitin B



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human ubiquitin (aa1-76) (Accession Nr. P0CG47) conjugated at the C-terminus to a quenched Rhodamine 110 dye.



Crossreactivity

Human



Application

Protein-based substrate. Typical working concentration range is 50-500nM. Hydrolysis of the conjugate results in fluorescence observable by excitation at 485nm and emission at 535nm.



Label/Conjugates

Rhodamine



MW

~9kDa



Purity

≥99% (LCMS)



Concentration

Lot dependent.



Accession Number

P0CG47



Formulation

Liquid. In 50mM MES pH 6.0.



Other Product Data

Click here for a Typical Lot-specific Product Datasheet from the Original Manufacturer
Our product description may differ slightly from the original manufacturers product datasheet.



Declaration

Manufactured by South Bay Bio.



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-80°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.
Protect from light.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin is a 76 amino acid post-translational modifier expressed throughout all tissues in eukaryotic organisms. The many roles of ubiquitin modification include proteasomal degradation, signal transduction, inflammatory response and DNA damage repair. Ubiquitin modification occurs through a pyramidal cascade of an E1 activating enzyme, E2 conjugating enzymes and an E3 ubiquitin ligase. This enzymatic cascade results in modification of a ε-amine of a lysine residue on a substrate protein. Substrates may either be mono- or poly-ubiquitinated by M1, K6, 11, 27, 29, 33, 48 or 63 linkages. Removal of ubiquitin from a substrate protein occurs via deconjugating enzymes, of which there are nearly 100 known enzymes with various specificities.