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商品編號


GWB-74034D



品牌


GenWay



公司


GenWay



公司分類


Secondary Antibodies



Size

1 mg

商品信息

Description:
Short Description:
DONKEY ANTI SHEEP/GOAT IgGFITC
Additional Information:




Name

Sheep/Goat IgG Antibody (FITC)



Related Product Names

DONKEY ANTI SHEEP/GOAT IgG: FITC IgGIGHG1



Gene Symbol

IGHG3



Gene Family

IGH



Gene id

3502



Alias Symbols

IgG3, FLJ39988, FLJ40036, FLJ40253, FLJ40587, FLJ40789, FLJ40834, MGC45809, DKFZp686H11213, IGHG3



Description of Target

DONKEY ANTI SHEEP/GOAT IgG:FITC



Swissprot ID

P01860



Species Reactivity

Sheep



Host

Donkey



Isotype

Polyclonal IgG



Conjugation

FITC



Replacement

This antibody may replace item sc-34663 from Santa Cruz Biotechnology.



Immunogen

Sheep IgG



Homology

Sheep



Product Format

Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid



Specificity

IgG



Concentration

1mg/ml



Applications

C



Application Info

Immunohistology - Frozen:
? ?1/100 - 1/200



Clonality

Polyclonal



Format

Solution: Phosphate buffered saline, Stabalizer: 0.09%, Sodium Azide. 1%, Bovine Serum Albumin, Form: Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid



St
ABI
lity

18 months from date of despatch.



Reconstitution and Storage

Store at +4
o
C or at -20
o
C if preferred.
This product should be stored undiluted.
Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.



Reactivity

Sheep



Storage

Store at +4°C or at -20°C if preferred. This product should be stored undiluted. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this



Protocol Information

Citation:
1: Hallstr?m T, Resman F, Ristovski M, Riesbeck K. Binding of complement regulators to invasive nontypeable Haemophilus influenzae isolates is not increased compared to nasopharyngeal isolates, but serum resistance is linked to disease severity. J Clin Microbiol. 2010 Mar;48(3):921-7. Epub 2010 Jan 20. PubMed PMID: 20089757; PubMed Central PMCID: PMC2832458.
Species:
Normal Human Serum(NHS)
Experiment Name:
Serum binding assay
Experiment Background:
In the present study, the characteristics of invasive nontypeable Haemophilus influenzae (NTHi) infections, including evidence of immune deficiency in the individual patient and the clinical presentation of the septic event, were studied. Teresia et al. correlated these findings with the capacity to bind specific complement regulators and the in vitro serum resistance of the individual isolates.
Experimental Steps:
1. To analyze whether NTHi from different isolation sites bound C4BP or factor H directly from NHS, bacteria were grown overnight in BHI broth. NTHi bacteria (109) were incubated with hiNHS and buffer (100 mM NaCl, 50 mM Tris-HCl, pH 7.4) for 1 h at37°C. 2. To remove unbound proteins, bacteria were washed 5 times with the same buffer. Thereafter, the bacterial pellet was res
USP
ended in 150 ?l of 0.1 M glycine-HCl, pH 2.0, in order to elute bound proteins.3. Bacteria without NHS were used as a negative control. After 15 min of incubation at 37°C with shaking, bacteria were centrifuged and the supernatants were subjected to SDS-PAGE (10%).4. To analyze whether NTHi bound vitronectin from NHS, bacteria were grown overnight and incubated with hiNHS and PBS. To removeunbound proteins, NTHi bacteria were washed 5 times with the same buffer.5. Thereafter, the bacterial pellet was res
USP
ended in 50 ?l of 0.1% Triton X-100 (Darmstadt, Germany) and protease inhibitors (Complete;
Roche
, Mannheim, Germany).6. After 30 min of incubation at 4°C, bacteria were centrifuged and the supernatants were subjected to SDS-PAGE (10%).7. Electrophoretic transfer of protein bands from the gel to an Immobilon-P membrane (
Millipore
, Bedford, MA) was done at 35 V for 2 h. After transfer, the Immobilon-P membrane was blocked in PBS with 0.1% Tween 20 (PBS-Tween) containing 5% milk powder. After several washings, the membrane was incubated with rabbit anti-human C4BP, goat anti-human factor H, or goat anti-human vitronectin pAb, followed by incubation with HRP-conjugated swine anti-rabbit or donkey-anti-goat pAb.8. After incubation and additional washings in PBS-Tween, development was performed with enhanced chemiluminescence (ECL) Western blotting detection reagents (
Pierce
, Rockford, IL).
Other Reagents Used:
NaCl, glucose, gelatin, MgCl2 , CaCl2.
Number Of Protocols:
1



Lead Time

Domestic: within 2-3 weeks delivery?International: 2-3 weeks



Intended Use

Research Use Only



Key Reference

1. Singh, M. et al. (1999) A recombinant measles virus expressing hepatitis B virus surface antigen induces humoral immune responses in genetically modified mice. J. Virol. 73 (6): 4823 - 4828.
2. Tedla, N. et al. (1998) Regulation of T lymphocyte trafficking into lymph nodes during an immune response by the chemokines macrophage inflammatory protein (MIP) - 1 alpha and MIP-1 beta. J. Immunol. 161: 5663 - 5672
3. Turner, J. et al. (2002) in vivo IL-10 production reactivates chronic pulmonary tuberculosis in C57BL/6 mice. J. Immunol. 169: 6343 - 6351.



::

Preservative St
ABI
lisers:
0.09% - Sodium Azide
1% - Bovine Serum Albumin
Antiserum Preparation:
Antisera to sheep IgG were raised by repeated immunisation of donkeys with highly purified antigen. Purified IgG was prepared by affinity chromatography.



::

Approx Protein Conc:
IgG concentration 1.0 mg/ml
Buffer Solutions:
Phosphate buffered saline pH7.2