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商品編號


AG-40T-0494-M001



品牌


Adipogen



公司


Adipogen



公司分類


Proteins



Size

1 mg

商品信息


More Information



Product Details



Synonyms

UBB; Ubiquitin B



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human ubiquitin K63R mutant (Accession Nr. P0CG47) fused to a N-terminal His-tag.



Crossreactivity

Human



MW

~8.5kDa



Purity

≥95% (SDS-PAGE)



Reconstitution

Soluble and stable aqueous buffers up to 10mg/ml.



Formulation

Lyophilized.



Other Product Data


Use:
Mutation of lysine 63 to arginine renders ubiquitin unable to form poly-ubiquitin chains via lysine 63 linkages with other ubiquitin molecules. Can form a ubiquitin-activating (E1) enzyme-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins. Ideal for the reduction in poly-ubiquitin chain length/conjugation rates and determining if poly-ubiquitin chains are K63 linked. Typical concentrations for non rate-limiting support of
in vitro
conjugation reactions range from 0.2-1mM depending on experimental conditions.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast ubiquitin, and 100% aa sequence identity shared between human and mouse ubiquitin. In mammals, four ubiquitin genes encode for two ubiquitin-ribosomal fusion proteins and two poly-ubiquitin proteins. Cleavage of the ubiquitin precursors by deubiquitinating enzymes gives rise to identical ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a ubiquitin-activating (E1) enzyme, a ubiquitin-conjugating (E2) enzyme, and a ubiquitin ligase (E3). ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of
BIOLOG
ical processes such as signal transduction, endocytosis, and post-endocytic sorting.