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商品編號


AG-40T-0498-M001



品牌


Adipogen



公司


Adipogen



公司分類


Proteins



Size

1 mg

商品信息


More Information



Product Details



Synonyms

UBB; Ubiquitin B



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human ubiquitin L8A mutant (Accession Nr. P0CG47).



Crossreactivity

Human



Formulation

Lyophilized from a solution in deionized water.



Other Product Data


Use:
Can form a ubiquitin-activating (E1) enzyme-catalyzed active thioester at the C-terminus allowing the molecule to be transferred to the lysines of substrate proteins. Can be conjugated to substrate proteins via the subsequent actions of a ubiquitin-activating (E1) enzyme, a ubiquitin-conjugating (E2) enzyme, and a ubiquitin ligase (E3). Chains consisting of mutant L8A are unable to interact with and be degraded by the 26S Proteasome. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.2-1mM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast ubiquitin, and 100% aa sequence identity shared between human and mouse ubiquitin. In mammals, four ubiquitin genes encode for two ubiquitin-ribosomal fusion proteins and two poly-ubiquitin proteins. Cleavage of the ubiquitin precursors by deubiquitinating enzymes gives rise to identical ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a ubiquitin-activating (E1) enzyme, a ubiquitin-conjugating (E2) enzyme, and a ubiquitin ligase (E3). ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of
BIOLOG
ical processes such as signal transduction, endocytosis, and post-endocytic sorting. Ubiquitin has a distinct and functionally important hydrophobic patch whose surface is defined by three residues including Leu8, Ile44 and Val70. These residues are solvent access
IBL
e in ubiquitin chains and are critical for poly-ubiquitin chain interaction and recognition by the 26S Proteasome and subsequent target degradation. These hydrophobic residues interact directly with various factors that bind to ubiquitin and/or mediate ubiquitin-ubiquitin interactions in ubiquitin chains that may influence orientation and recognition.