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商品編號


AG-40T-0503F-C050



品牌


Adipogen



公司


Adipogen



公司分類


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

UBB; Ubiquitin B



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human ubiquitin (Accession Nr. P0CG47) conjugated to a N-terminal FITC (single modification).



Crossreactivity

Human



Label/Conjugates

FITC



Formulation

Lyophilized from a solution in deionized water.



Other Product Data


Use:
Produced via a proprietary process resulting in a single fluorescein modification exclusively on the N-terminus of ubiquitin. This site-specific modification results in an ubiquitin that is fully functional at the C-terminus, and with the full compliment of reactive lysines to allow for polyubiquitin chain incorporation. This reagent allows for poly-ubiquitin chain incorporation of fluorescein-N-terminal ubiquitin with higher efficiency and detection sensitivity than tr
ADI
tionally modified ubiquitins. Ideal for use in assays requiring fluorescent detection. Optimal fluorescence at pH 8.0 is monitored with an excitation wavelength of 490nM and an emission wavelength of 515nM. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-1?M.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast ubiquitin, and 100% aa sequence identity shared between human and mouse ubiquitin. In mammals, four ubiquitin genes encode for two ubiquitin-ribosomal fusion proteins and two poly-ubiquitin proteins. Cleavage of the ubiquitin precursors by deubiquitinating enzymes gives rise to identical ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a ubiquitin-activating (E1) enzyme, a ubiquitin-conjugating (E2) enzyme, and a ubiquitin ligase (E3). ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of
BIOLOG
ical processes such as signal transduction, endocytosis, and post-endocytic sorting.