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商品編號


GWB-7B623E



品牌


GenWay



公司


GenWay



公司分類


Secondary Antibodies



Size

1 ml

商品信息

Additional Information:




Name

Donkey Anti-Rat IgG (H+L)



Related Product Names

Donkey Anti-Rat IgG(H+L), Mouse ads-AP; N/A; N/A Donkey Anti-Rat IgG (H+L)



Gene Symbol

IGHG1



Gene Fullname

Ig gamma-1 chain C region



Protein Name

Ig gamma-1 chain C region



Swissprot ID

P01857



Host

Pooled antisera from donkey hyperimmunized with rat IgG.



Isotype

IgG



Replacement

This antibody may replace item sc-52003 from Santa Cruz Biotechnology.



NCBI Acc Number

CAA10831.1



Product Format

Alkaline Phosphatase (AP) Conjugate



Purification

Affinity chromatography on Rat IgG covalently linked to agarose.



Specificity

Reacts with the heavy and light chains of Rat IgG as demonstrated by
ELISA
and/or FLISA. May also react with the light chains of other Ratimmunoglobulins.



Applications

IF,
ELISA
, WB, DB, IHC



Application Info

- Immunofluorescent staining
- Enzyme-Linked-Immunosorbent-Assay (
ELISA
)
- Fluorescent-Linked-Immunosorbent-Assay (FLISA)
- Western blotting
- Dot-and slot-immunoblotting
- Immunohistochemistry



GI Number

2632200



Dilution

FLISA:
ELISA
:
FITC conjugate
R-PE conjugate
AP conjugate
HRP conjugate
BIOT conjugate
1:200 – 1:400
1ug
1:2,000-1:4,000
1:4,000-1:8,000
1:5,000-1:20,000



Clonality

Polyclonal



Format

Alkaline Phosphatase (AP) Conjugate



Absorption

Minimal cross reaction to mouse serum proteins



Reactivity

Rat



Sequence Length

130



Warning

Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing.



Storage

The alkaline phosphatase (AP) conjugate is supplied as 1.0mL stock solution in 50mM Tris/1mM MgCl
2
/50% Glycerol, pH 8.0, containing 0.1% NaN
3
as preservative. Store at 2-8C long-term at -20C



Protocol

Reconstitution Instructions
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme- Linked ImmunoSorbent Assay (
ELISA
) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol



Lead Time

Domestic: within 1-2 weeks delivery?International: 1-2 weeks



Intended Use

Research Use Only