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商品編號(hào)


AG-40T-0514B-C050



品牌


Adipogen



公司


Adipogen



公司分類(lèi)


Proteins



Size

50 ?g

商品信息


More Information



Product Details



Synonyms

UBB; Ubiquitin B



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human ubiquitin (Accession Nr. P0CG47) conjugated to a N-terminal biotin (single modification). All reactive lysine residues which have been mutated to arginine.



Crossreactivity

Human



Label/Conjugates

Biotin



Formulation

Lyophilized.



Other Product Data


Use:
Produced via a proprietary process resulting in a single Biotin modification exclusively on the N-terminus of ubiquitin. This site-specific modification results in a ubiquitin that is fully functional at the C-terminus. This ubiquitin mutant lacks all reactive lysine residues which have been mutated to arginine. These mutations render the protein unable to form poly-ubiquitin chains and can be used as a negative control or to detect mono-ubiquitination. Poly-ubiquitin chain visualization/quantitation can be performed via avidin-linked detection allowing for a higher efficiency and detection sensitivity than with anti-ubiquitin antibodies. Ideal as an alternative to r
ADI
o-labeled ubiquitin. Depending on desired signal strength and assay conditions, Biotin-N-term-ubiquitin should be used in conjunction with native ubiquitin at combined concentration range of 10-50μM with a 1:2 to 1:20 ratio of Biotin-N-term-ubiquitin: native ubiquitin, respectively. Typical amounts for a 20μl reaction: 250ng to 2?g Biotin-N-term-ubiquitin, 5?g native ubiquitin.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

BLUE ICE



Short Term Storage

+4°C



Long Term Storage

-20°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -20°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast ubiquitin, and 100% aa sequence identity shared between human and mouse ubiquitin. In mammals, four ubiquitin genes encode for two ubiquitin-ribosomal fusion proteins and two poly-ubiquitin proteins. Cleavage of the ubiquitin precursors by deubiquitinating enzymes gives rise to identical ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a ubiquitin-activating (E1) enzyme, a ubiquitin-conjugating (E2) enzyme, and a ubiquitin ligase (E3). ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of
BIOLOG
ical processes such as signal transduction, endocytosis, and post-endocytic sorting.