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商品編號(hào)


AG-40T-0517-C025



品牌


Adipogen



公司


Adipogen



公司分類


Proteins



Size

25 ?g

商品信息


More Information



Product Details



Synonyms

Ub-VS



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human ubiquitin (Accession Nr. P0CG47) tagged to vinyl sulfone fused to a N-terminal HA-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM MES, pH 6.0.



Other Product Data


Use:
Potent, irrevers
IBL
e and specific inhibitor of most deubiquitinating enzymes (DUBs) including ubiquitin C-terminal hydrolases (UCHs) and ubiquitin-specific proteases (
USP
s). Useful for inhibiting the hydrolysis of poly-ubiquitin chains on substrate proteins
in vitro
and thus enhances poly-ubiquitin chain accumulation. The HA-tag allows for detection and purification of deubiquitinating enzymes activity. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 1-5?M for
in vitro
assays to inhibit deubiquitinating enzymes.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin is a 76 amino acid (aa) protein that is ubiquitously expressed in all eukaryotic organisms. ubiquitin is highly conserved with 96% aa sequence identity shared between human and yeast ubiquitin, and 100% aa sequence identity shared between human and mouse ubiquitin. In mammals, four ubiquitin genes encode for two ubiquitin-ribosomal fusion proteins and two poly-ubiquitin proteins. Cleavage of the ubiquitin precursors by deubiquitinating enzymes gives rise to identical ubiquitin monomers each with a predicted molecular weight of 8.6 kDa. Conjugation of ubiquitin to target proteins involves the formation of an isopeptide bond between the C-terminal glycine residue of ubiquitin and a lysine residue in the target protein. This process of conjugation, referred to as ubiquitination or ubiquitylation, is a multi-step process that requires three enzymes: a ubiquitin-activating (E1) enzyme, a ubiquitin-conjugating (E2) enzyme, and a ubiquitin ligase (E3). ubiquitination is classically recognized as a mechanism to target proteins for degradation and as a result, ubiquitin was originally named ATP-dependent Proteolysis Factor 1 (APF-1). In addition to protein degradation, ubiquitination has been shown to mediate a variety of
BIOLOG
ical processes such as signal transduction, endocytosis, and post-endocytic sorting.