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商品編號


AG-40T-0528-C100



品牌


Adipogen



公司


Adipogen



公司分類


Proteins



Size

100 ?g

商品信息


More Information



Product Details



Synonyms

Ubiquitin-fold Modifier Conjugating Enzyme 1; HSPC155



Product Type

Protein



Properties



Source/Host

E. coli



Sequence

Human Ufc1 (Accession Nr. Q9Y3C8) fused to a N-terminal His-tag.



Crossreactivity

Human



Formulation

Liquid. In 50mM HEPES pH 7.5, 200mM NaCl, 10% Glycerol (v/v), 2mM TCEP.



Other Product Data


Use:
Member of the UFM1-conjugating (E2) enzyme family that receives UFM1 from a UFM1-activating (E1) enzyme and subsequently interacts with a UFM1 ligase (E3) to conjugate UFM1 to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial protein concentration of 0.1-?1μM.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S

No



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin-fold Modifier Conjugating Enzyme 1 (Ufc1) has been identified as an E2-like conjugating enzyme for ubiquitin fold modifier 1 (Ufm1). Ufc1 is a 116 amino acid (aa) protein with a predicted molecular weight of 19 kDa. Human Ufc1 exhibits 97% and 98% aa identity to its mouse and rat orthologs, respectively. The process of attaching Ufm1 to target proteins is termed ufmylation. It is thought to occur in a manner analogous to the ubiquitin system, including stepwise enzymatic reactions involving ubiquitin-activating (E1)-, ubiquiting-conjugating (E2)-, and ubiquitin Ligase (E3)-like enzymes. In addition to Ufc1, other members of the Ufm1-conjugation system include the E1-like enzyme Uba5, E3-like enzyme Ufl1, and deconjugating enzymes UfSP1 and UfSP2.