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商品編號(hào)


AG-40T-0571-1



品牌


Adipogen



公司


Adipogen



公司分類


Proteins



Size

1 Vial


商品信息


More Information



Product Details



Synonyms

Ubiquitin Carboxyl-terminal Hydrolase 12; Deubiquitinating Enzyme 12; Ubiquitin Thioesterase 12; Ubiquitin-specific-processing Protease 12;
USP
1-associated Factor 1; WD Repeat-containing Protein 48



Product Type

Protein



Properties



Source/Host

Sf21 cells



Sequence

Human
USP
12 (Accession Nr. NP_872294.2).
Human UAF1 (Accession Nr. NP_001333157.1).



Crossreactivity

Human



MW

~44kDa & ~77kDa



Purity

≥95% (SDS-PAGE)



Formulation

Liquid. In 50mM HEPES, pH8.0, 150mM NaCl, 10% glycerol, 2mM TCEP.



Other Product Data


Use:
Reaction conditions will need to be optimized for each specific application. We recommend an initial recombinant human
USP
12/UAF1 concentration of 50-500 nM when using Ubiquitin-AMC (AG-40T-0459) or Ubiquitin-Rh110 (AG-40T-0459RH) substrates.
In vitro
, the
USP
12/UAF1 complex will not efficiently hydrolyze poly-Ubiquitin chains in the absence of WDR20.



Declaration

Manufactured by Boston Biochem



Shipping and Handling



Shipping

DRY ICE



Short Term Storage

-20°C



Long Term Storage

-80°C



Handling Advice

Aliquot to avoid freeze/thaw cycles.



Use/St
ABI
lity

Stable for at least 1 year after receipt when stored at -80°C.



Documents



MSD
S


Download PDF



Product Specification Sheet



Datasheet


Download PDF





Ubiquitin carboxyl-terminal hydrolase 12 (
USP
12) is a specialized cysteine protease with a predicted molecular weight of 43 kDa.
USP
12 is a member of the peptidase C19 family and
USP
12/
USP
46 subfamily, and the human protein shares 98% amino acid sequence identity with its mouse ortholog. Like
USP
1 and
USP
46,
USP
12 lacks appreciable activity in the absence of its co-activator UAF1. Addition of UAF1 increases the in vitro activity of
USP
12 20-fold or more. WDR20 is often found in complex with both
USP
12 and UAF1, and the ternary complex is reported to be more active than the
USP
12/UAF1 complex.
BIOLOG
ically,
USP
12 has been implicated as a positive regulator of Androgen Receptor (AR) activity via a mechanism that involves down-regulation of Akt activity. As such, it has been proposed that
USP
12 inhibition could offer a therapeutic target for some forms of prostate cancer. Recombinant
USP
12 contains a C-terminal 6-His tag. Recombinant UAF1 also contains a C-terminal 6-His tag. The assembled complex contains 25 μg
USP
12 and 45 μg UAF1, resulting in a 1:1 stoichiometry.