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商品編號


EXOEL-CD9A-1



品牌


SBI



公司


System Biosciences（SBI）



公司分類(lèi)


exosome quantitation




商品信息

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Overview:
Sensitive,
ELISA
-based exosome quantitation

When you would like to use an
ELISA
-based method to find out how many exosomes you have and time is not limiting, choose one of
SBI
’s Exo
ELISA
Complete Kits. Calibrated using NanoSight Analysis, the Exo
ELISA
Complete Kit (CD9 Detection) enables calculation of exosome abundance based on the presence of CD9, a general exosome
Marker
. Note that our newer Exo
ELISA
-ULTRA Complete Kit (CD63 Detection) can provide faster exosome quantitation using significantly less sample.



Sensitive—detect as little as 500 ?g protein equivalent

Flex
IBL
e—compat
IBL
e with all major exosome isolation methods (
e.g.
ExoQuick
?
, ultracentrifugation, ultrafiltration, and immunoaffinity capture) from human, mouse, and rat

Quantitative—calibrated internal standards enable quantitation of exosomes carrying CD9


Choose the exosome quantitation method that’s best for your studies




?

Exo
ELISA
-ULTRA CD63 Detection

Exo
ELISA
CD9
Exo
ELISA
CD63
Exo
ELISA
CD81

EXOCET

FluoroCet





Use

For fast and sensitive antibody-based quantitation of exosomes

For sensitive quantitation of exosomes when time and input sample are not limiting

For fast quantitation of extracellular vesicles with moderate sample input requirements

For the most sensitive quantitation of extracellular vesicles with very low sample input requirements



Detection method

Antibody

Antibody

Enzymatic

Enzymatic



Quantitation chemistry

Enzymatic (HRP)

Enzymatic (HRP)

Colorimetric

Fluorescent



Total protocol time

4 hours (no overnight incubation)

24 hours

20 min

60 min



Input sample amount (protein equivalent)

1 – 200 ?g

>500 ?g

50 ?g

<1 ?g









ExoELISA-ULTRA CD63 Detection

ExoELISA CD9
ExoELISA CD63
ExoELISA CD81

EXOCET

FluoroCet



Use

For fast and sensitive antibody-based quantitation of exosomes

For sensitive quantitation of exosomes when time and input sample are not limiting

For fast quantitation of extracellular vesicles with moderate sample input requirements

For the most sensitive quantitation of extracellular vesicles with very low sample input requirements



Detection method

Antibody

Antibody

Enzymatic

Enzymatic



Quantitation chemistry

Enzymatic (HRP)

Enzymatic (HRP)

Colorimetric

Fluorescent



Total protocol time

4 hours (no overnight incubation)

24 hours

20 min

60 min



Input sample amount (protein equivalent)

1 – 200 ?g

>500 ?g

50 ?g

<1 ?g




How It Works:
Our ExoELISA Kits have all the reagents you need to run the ELISA—just add lysed exosome particles. The kits are compat
IBL
e with exosomes isolated using most methods, including ExoQuick
?
, ExoQuick-TC
?
, or ultracentrifugation.



The lysed exosome particles (and, thus, exosomal proteins) are directly immobilized onto the wells of the microtiter plate, and after binding, a blocking agent is added to prevent non-specific binding of the primary detection antibody, anti-CD9. Following addition of anti-CD9, a secondary antibody (goat anti-rabbit) linked to horser
ADI
sh peroxidase (HRP) is also added to amplify the signal and increase assay sensitivity.

The amount of CD9 is measured via activity of the bound HRP-secondary antibody using a colorimetric assay with extra-sensitive TMB as the substrate. The accumulation of colored product is proportional to the amount of CD9 present in each well, and is measured using a microtiter plate reader at 450 nm absorbance.

Each ExoELISA Kit includes a set of standards calibrated to a known amount of exosome particles as determined by NanoSight analysis. These standards can be used to generate a calibration curve enabling quantitation of exosomes carrying CD9 from the ExoELISA data.
Citations:
Woo, HK, et al. (2017) Exodisc for Rapid, Size-Selective, and Efficient Isolation and Analysis of Nanoscale Extracellular Vesicles from
BIOLOG
ical Samples.
ACS Nano
. 2017 Feb 28; 11(2):1360-1370. PM ID: 28068467
Galazka, G, et al. (2017) Multiple sclerosis: Serum-derived exosomes express myelin proteins.
Mult. Scler.
. 2017 Feb 1;:1352458517696597. PM ID: 28273783
Hurwitz, SN, et al. (2017) CD63 Regulates Epstein-Barr Virus LMP1 Exosomal Packaging, Enhancement of Vesicle Production, and Noncanonical NF-κB Signaling.
J. Virol.
. 2017 Mar 1; 91(5). PM ID: 27974566
Desgagné, V, et al. (2017) Changes in high-density lipoprotein-carried miRNA contribution to the plasmatic pool after consumption of dietary trans fat in healthy men.
Epigenomics
. 2017 May 1; 9(5):669-688. PM ID: 28470118
Patel, DB, Gray, KM & Santharam, Y. (2017) Impact of Cell Culture Parameters on Production and Vascularization Bioactivity of Mesenchymal Stem Cell‐Derived Extracellular Vesicles.
Bioengineering & Translational Medicine
. 2017 Jun 26;. Link: Bioengineering & Translational Medicine
Bidarimath, M, et al. (2017) Extracellular vesicle mediated intercellular communication at the porcine maternal-fetal interface: A new par
ADI
gm for conceptus-endometrial cross-talk.
Sci Rep
. 2017 Jan 12; 7:40476. PM ID: 28079186
Nocera, AL, et al. (2017) Exosomes mediate interepithelial transfer of functional P-glycoprotein in chronic rhinosinusitis with nasal polyps.
Laryngoscope
. 2017 May 9;. PM ID: 28485529
Mead, B & Tomarev, S. (2017) Bone Marrow-Derived Mesenchymal Stem Cells-Derived Exosomes Promote Survival of Retinal Ganglion Cells Through miRNA-Dependent Mechanisms.
Stem Cells Transl Med
. 2017 Apr 1; 6(4):1273-1285. PM ID: 28198592
Elshelmani, H & Rani, S. (2017) Exosomal MicroRNA Discovery in Age-Related Macular Degeneration.
Methods Mol. Biol.
. 2017 Nov 9; 1509:93-113. PM ID: 27826921
Tao, SC, et al. (2017) Chitosan Wound Dressings Incorporating Exosomes Derived from MicroRNA-126-Overexpressing Synovium Mesenchymal Stem Cells Provide Sustained Release of Exosomes and Heal Full-Thickness Skin Defects in a Diabetic Rat Model.
Stem Cells Transl Med
. 2017 Mar 1; 6(3):736-747. PM ID: 28297576
Li, Y, et al. (2017) Comparative Gene Expression Analysis of Lymphocytes Treated with Exosomes Derived from Ovarian Cancer and Ovarian Cysts.
Front Immunol
. 2017 Jun 16; 8:607. PM ID: 28620375
Barclay, RA, et al. (2017) Isolation of Exosomes from HTLV-Infected Cells.
Methods Mol. Biol.
. 2017 Mar 30; 1582:57-75. PM ID: 28357662
Xia, Y, et al. (2017) A vis
IBL
e and colorimetric aptasensor based on DNA-capped single-walled carbon nanotubes for detection of exosomes.
Biosens Bioelectron
. 2017 Jun 15; 92:8-15. PM ID: 28167415
Tan, DB, et al. (2017) Elevated levels of circulating exosome in COPD patients are associated with systemic inflammation.
Respiratory Medicine
. 2017 Apr 26;. Link: Respiratory Medicine
Guo, SC, et al. (2017) Exosomes derived from platelet-rich plasma promote the re-epithelization of chronic cutaneous wounds via activation of YAP in a diabetic rat model.
Theranostics
. 2017 Jan 2; 7(1):81-96. PM ID: 28042318
Erkan, EP, et al. (2017) Extracellular vesicle-mediated suicide mRNA/protein delivery inhibits glioblastoma tumor growth in vivo.
Cancer Gene Ther.
. 2017 Jan 1; 24(1):38-44. PM ID: 27982017
Protocol, IIEI. (2017) List of Components.
Product
. ;. Link: Product
Tao, SC, et al. (2017) Exosomes derived from miR-140-5p-overexpressing human synovial mesenchymal stem cells enhance cartilage tissue regeneration and prevent osteoarthritis of the knee in a rat model.
Theranostics
. 2017 Jan 2; 7(1):180-195. PM ID: 28042326
Gutierrez, S, et al. (2017) A fast, effective, alternative method for exosome isolation from cell culture media.
Poster
. ;. Link: Poster
Banton, S. (2017) Human peripheral reticulocyte isolation and exosome release in vitro.
Thesis
. ;. Link: Thesis