SBI/pCT-CD63-RFP (pCMV, Exosome/Secretory, CD63 Tetraspanin Tag; pre-packaged virus)/CYTO120R-VA-1/>2x10^6 IFUs
基因編輯
商品編號
CYTO120R-VA-1
品牌
SBI
公司
System Biosciences(SBI)
公司分類(lèi)
exosome labeling
商品信息
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Overview:
Easily track exosomes with a CD63-RFP fusion
Monitor exosome dynamics with the pCT-CD63-RFP (pCMV) Exosome Cyto-Tracer (also available as a CD63-GFP fusion, pCT-CD63-GFP). Provided as either lentivector or pre-packaged virus, this Cyto-Tracer fuses RFP to the tetraspanin CD63, which then gets packaged into exosomes produced by the transfected cells. Great for creating stable cell lines that produce RFP-labeled exosomes as well as labeling exosomes from primary cells, tumor cells, stem cells, and more.
Stable lentivector-based system
Ideal for co-localization studies
Monitor exosome dynamics and functional studies in real time
Label exosomes from primary cells, tumor cells, stem cells, and more
Choose the exosome quantitation method that’s best for your studies
Promoter (Cat.#)
Cyto-Tracer
Target Location
Peptide Tag
CMV (CYTO100-PA-1/CYTO100-VA-1)
MSCV (CYTO100-PB-1/CYTO100-VB-1)
pCT-Mem-GFP
Plasma Membrane
Neuromodulin
CMV (CYTO101-PA-1/CYTO101-VA-1)
MSCV (CYTO101-PB-1/CYTO101-VB-1)
pCT-InnerMem-GFP
Inner Leaflet of Membrane
Farnesylation signal
CMV (CYTO102-PA-1/CYTO102-VA-1)
MSCV (CYTO102-PB-1/CYTO102-VB-1)
pCT-Mito-GFP
Mitochondria
Cytochrome C oxidase VIII
CMV (CYTO103-PA-1/CYTO103-VA-1)
pCT-ER-GFP
Endoplasmic Reticulum
ER targeting consensus
CMV (CYTO104-PA-1/CYTO104-VA-1)
pCT-Golgi-GFP
Golgi Apparatus
Galactosyltrasferase
CMV (CYTO105-PA-1/CYTO105-VA-1)
pCT-Secretory-GFP
ER-Golgi? Vesicles
Secretory consensus peptide
CMV (CYTO106-PA-1/CYTO106-VA-1)
pCT-Lyso-GFP
Lysosome
Cathepsin B
CMV (CYTO107-PA-1/CYTO107-VA-1)
pCT-Endo-GFP
Endosome
RhoB
CMV (CYTO107R-PA-1/CYTO107R-VA-1)
pCT-Endo-RFP
Endosome
RhoB
CMV (CYTO108-PA-1/CYTO108-VA-1)
MSCV (CYTO108-PB-1/CYTO108-VB-1)
pCT-Pero-GFP
Peroxisome
Peroxisomal consensus signal
CMV (CYTO120-PA-1/CYTO120-VA-1)
pCT-Autophago-GFP
Autophagosome
LC3
CMV (CYTO120R-PA-1/CYTO120R-VA-1)
pCT-Autophago-RFP
Autophagosome
LC3
CMV (CYTO109-PA-1/CYTO109-VA-1)
pCT-CD63-GFP
Exosome/Secretory
CD63 Tetraspanin
CMV (CYTO109R-PA-1/CYTO109R-VA-1)
pCT-CD63-RFP
Exosome/Secretory
CD63 Tetraspanin
CMV (CYTO122-PA-1/CYTO122-VA-1)
pCT-CD9-GFP
Exosome/Secretory
CD9 Tetraspanin
CMV (CYTO123-PA-1/CYTO123-VA-1)
pCT-CD9-RFP
Exosome/Secretory
CD9 Tetraspanin
CMV (CYTO124-PA-1/CYTO124-VA-1)
pCT-CD81-GFP
Exosome/Secretory
CD81 Tetraspanin
CMV (CYTO125-PA-1/CYTO125-VA-1)
pCT-CD81-RFP
Exosome/Secretory
CD81 Tetraspanin
CMV (CYTO110-PA-1/CYTO110-VA-1)
MSCV (CYTO110-PB-1/CYTO110-VB-1)
pCT-Actin-GFP
Cytoskeleton
β-actin
CMV (CYTO111-PA-1/CYTO111-VA-1)
MSCV (CYTO111-PB-1/CYTO111-VB-1)
pCT-Tublin-GFP
Cytoskeleton
α-tublin
CMV (CYTO111R-PA-1/CYTO111R-VA-1)
pCT-Tublin-RFP
Cytoskeleton
α-tublin
CMV (CYTO112-PA-1/CYTO112-VA-1)
pCT-MAP4-GFP
Microtubule
Microtubule associated protein 4 (MAP4)
CMV (CYTO113-PA-1/CYTO113-VA-1)
MSCV (CYTO113-PB-1/CYTO113-VB-1)
pCT-H2B-GFP
Nucleus
H2B
CMV (CYTO114-PA-1/CYTO114-VA-1)
pCT-Apoptosis-Luc
?Caspase 3/7 activation
Cyclic inactive luciferase
CMV (CYTO115-PA-1/CYTO115-VA-1)
pCT-GFP-BAX
Cytosol to Mitochondria
BAX
CMV (CYTO116-PA-1/CYTO116-VA-1)
pCT- Catenin-GFP
Cytosol to Nucleus/Cell Membrane
β-Catenin activation
CMV (CYTO117-PA-1/CYTO117-VA-1)
pCT-CMV-PSD95-GFP
Dendrite Membranes
PSD-95
CMV (CYTO118-PA-1/CYTO118-VA-1)
MSCV (CYTO118-PB-1/CYTO118-VB-1)
pCT-Cyto-GFP
Cytosolic Compartment
GFP-Untagged
CMV (CYTO119-PA-1/CYTO119-VA-1)
pCT-Cyto-RFP
Cytosolic Compartment
RFP-Untagged
MSCV (CYTO121-PB-1/CYTO121-VB-1)
pCT-Cyto-GFpLuc
GFP and Luciferase Control
GFP and Luciferase (Untagged)
Supporting Data:
See some of our exosome Cyto-Tracers in action
The following figure and videos are from:
Garcia NA,
et al
. Glucose Starvation in Cardiomyocytes Enhances Exosome Secretion and Promotes Angiogenesis in Endothelial Cells.
PLoS ONE
. 2015.
10
(9). PMCID: PMC4578916.
Figure 4 from Garcia,
et al
. Exosome transfer from CMs to ECs.
(A) H9c2 transfected with pCT-CD63-GFP. FACS analysis of 90% GFP positive cells. (B) Representative images from confocal time-lapse microscopy of mouse ECs (ACTB-DsRed) (EC; red) co-cultured with H9C2-CD63-GFP cells (green), previously cultured for 24 h in +/-St medium. Exosome transfer from H9C2 CMs to EC can be observed (S1 and S2 Movies, below). White arrows show CD63-GFP structures inside ECs (C) Representative immunostaining of H9C2-CD63-GFP and HUVEC co-cultures; anti-GFP (green) and anti-CD31 (red). The images illustrate GFP fluorescence from CD63-GFP exosomes in CD31-positive cells (red) after 24 h incubation in +/-St medium. White arrows show CD63-GFP structures inside ECs.
Video S1 from Garcia,
et al
. Exosome transfer from H9C2-CD63-GFP (green) to endothelial DsRed cells (red) under +St conditions.
Video S2 from Garcia,
et al
. Exosome transfer from H9C2-CD63-GFP (green) to endothelial DsRed cells (red) under -St conditions.
Labeled exosomes.
(Top panels) CD63-GFP Cyto-Tracers transfected into a human fibrosarcoma cell line. (Bottom panels) CD9-GFP and CD9-RFP Cyto-tracers co-transfected into HEK293 cells.
Citations:
Braganza, A, et al. (2017) UBE3B Is a Calmodulin-regulated, Mitochondrion-associated E3 Ubiquitin Ligase.
J. Biol. Chem.
. 2017 Feb 10; 292(6):2470-2484. PM ID: 28003368
Gonzalez-King, H, et al. (2017) Hypoxia Induc
IBL
e Factor-1α Potentiates Jagged 1-Mediated Angiogenesis by Mesenchymal Stem Cell-Derived Exosomes.
Stem Cells
. 2017 Jul 1; 35(7):1747-1759. PM ID: 28376567
Jawhari, S, et al. (2017) Autophagy and TrkC/NT-3 signaling joined forces boost the hypoxic glioblastoma cell survival.
Carcinogenesis
. 2017 Jun 1; 38(6):592-603. PM ID: 28402394
Soares, VYR. (2017) O papel das vesículas extracelulares na fisiopatologia da perda auditiva ocasionada pelo Schwannoma vestibular.
Thesis
. ;. Link: Thesis
Yoon, C, et al. (2016) Delivery of miR-155 to retinal pigment epithelial cells mediated by Burkitt’s lymphoma exosomes.
Tumour Biol.
. 2016 Jan 1; 37(1):313-21. PM ID: 26211004
Jawhari, S. (2015) Etude des signalisations autophagique et neurotrophique dans des lignées de glioblastome humain activées lors de l’hypoxie.
Thesis
. ;. Link: Thesis
Lo Cicero, A, et al. (2015) Exosomes released by keratinocytes modulate melanocyte pigmentation.
Nat Commun
. 2015 Jun 24; 6:7506. PM ID: 26103923
VARLET, P, PU-PH, P & DJAVAHERI-MERGNY, M. (2015) SOHA JAWHARI.
Thesis
. ;. Link: Thesis
Liang, Y. (2015) Exosome/Microvesicle Cargo Contents Recruitment in a Glycosylation-dependent Pathway.
Thesis
. ;. Link: Thesis
Sato, Y, et al. (2014) MTCL1 crosslinks and st
ABI
lizes non-centrosomal microtubules on the Golgi membrane.
Nat Commun
. 2014 Nov 4; 5:5266. PM ID: 25366663
Liang, Y, et al. (2014) Complex N-linked glycans serve as a determinant for exosome/microvesicle cargo recruitment.
J. Biol. Chem.
. 2014 Nov 21; 289(47):32526-37. PM ID: 25261472
Fang, Q, et al. (2014) HSP90 regulates DNA repair via the interaction between XRCC1 and DNA polymerase β.
Nat Commun
. 2014 Nov 26; 5:5513. PM ID: 25423885
產(chǎn)品貨號:7079.2