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SBI/ExoGlowTM-Membrane EV Labeling Kit/EXOGM600A-1/20 Reactions

基因編輯
SBI/ExoGlowTM-Membrane EV Labeling Kit/EXOGM600A-1/20 Reactions


商品編號


EXOGM600A-1



品牌


SBI



公司


System Biosciences(SBI)



公司分類(lèi)


exosome labeling



商品信息

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Overview:
See EVs better with reagents specifically optimized for labeling

SBI
’s new generation of extracellular vesicle (EV) labeling reagents take your EV visualization to new levels of clarity, with low background and high selectivity. Unlike general-purpose labeling reagents that are not optimized for EVs and suffer from high levels of background signal, ExoGlow
TM
-Membrane improves your
ABI
lity to track and localize EVs by specifically labeling EV membranes with a proprietary fluorescent dye that delivers very low levels of background signal. The result is unmatched EV imaging for more accurate studies (see below for our full range of next generation ExoGlow reagents).



Specific—carefully developed to generate a robust signal specific for EV membranes, le
ADI
ng to very low levels of background

Compat
IBL
e—delivers robust performance on EVs isolated using all methods tested—including ExoQuick
?
, ultracentrifugation, and column-based workflows

Easy-to-use—labeling protocol is quick and straightforward

Powerful—can be used with as little as 1 ?g of EVs


ExoGlow-RNA properties:
Excitation: 465 nm
Emission: 635 nm
Laser line: 488 nm

Available next generation ExoGlow EV imaging reagents




EV Component Labeled

Cat. #

Product

xcitation (nm)

Emission (nm)

Laser Line (nm)





Internal protein

EXOGP100A-1

ExoGlow
?
-Protein EV Labeling Kit (Red)

573

588

561



EXOGP300A-1

ExoGlow
?
-Protein EV Labeling Kit (Green)

511

525

488



EXOGP400A-1

ExoGlow
?
-Protein EV Labeling Kit (Blue)

403

454

405



RNA

EXOGR800A-1

ExoGlow
?
-RNA EV Labeling Kit

485

537

488



Membrane

EXOGM600A-1

ExoGlow
?
-Membrane EV Labeling Kit

465

635

488





Supporting Data:

Imaging EVs with a new level of clarity



Figure 1. ExoGlow-Membrane enables clear visualization of labeled EVs being internalized by target cells.
We labeled HEK293T EVs with ExoGlow-Membrane and followed uptake by HEK293T cells. The evenly distributed fluorescence signal shows internalization of labeled EVs by the target cells and the distribution of EV membranes to cellular membranes.


Figure 2. ExoGlow-Membrane is specific for intact membranes.
We incubated either PBS,
liposomes
,
liposomes
pre-solubilized with detergent (0.05% Tx100), EVs, or lipoproteins. Only the samples with intact membranes—
liposomes
and EVs (blue)—show a high fluorescence signal, demonstrating ExoGlow-Membrane’s specificity for intact membranes.



上一篇 SBI/ExoGlowTM-Protein EV Labeling Kit (Red)/EXOGP100A-1/20 Reactions  下一篇 SBI/ExoGlowTM-RNA EV Labeling Kit/EXOGR800A-1/10 Reactions

產(chǎn)品貨號:3204.0

3204.0 ¥
11至15個(gè)工作日送達
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